Abstract:Phenol pollution in the environment mainly referred to phenolic compounded on water pollution, waste water containing phenol was usually turned around the highest levels of phenol and cresol. Phenol was widespread in the petroleum, chemical, gas, coke, steel and phenolic wastewater plant emissions. Phenolic wastewater emissions of water pollution, it not only poisoned fish, but also damaged crops, and a serious threat to human health, water pollution control in China has been a key to solve one of the harmful waste.
In this experiment, wastewater was extracted from Xuzhou Institute of technology south campus of the sludge in the screening of phenol-degrading strains, and made further evaluation on bacterial strain, physiology and biochemistry.
It showed that this bacterial strain had moveability and nead oxygen through its physiological and biochemical research;Catalase test showed that the bacteria was gram-positive bacterium and could produce catalase;Methyl red experiment result showed that the bacteria could decompose glucose and produce large amount of acid products , enable Methyl red indicator turned to red and showed positive; Tween80 degradation experiment and tolerance test of 0.1% Congo red result also presented positive.
At last, the strain was finally identified as Pseudomonas through the 16S rDNA identification.
Keywords: Phenol ; Screening; Identification; Physiological and biochemical properties; Pseudomonas